farther study in green and black tea should be done particularly antimicrobial study.
References
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(2) Chiu, A, E, Chan M.C.and Kern D.G. (2005). Double –blinded, placebo- controlled trial of green tea extracts in the clinical and histological appearance of photo aging skin.Dermatol Slurg. 31(7 pt 2):855 – 860.
(3) Chow H.H., Hakim, I.A., Vining D.R. (2005).Effects of dosing condition on the oral bioavailability of green tea catechins after single=dose administration of polyphenols in healthy individuals. Clin. Cancer Res. 11(12):4627-4633.
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(8) AOAC. 1990. Association of Official Analytical Chemists. Official Methods of Analysis. 15th edition. Washington.
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Table (1): Chemical constituents that available (+ ve) and non-available (- ve) in green and black tea.
Solvent extraction /
Compounds
Water extraction
Ethanol extraction
Petroleum ether extraction
Green tea
Black tea
Green tea
Black tea
Green tea
Black tea
1/Alkaloid
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
2/Anthraquinones
(-) ve
(-) ve
(-) ve
(-) ve
(-) ve
(-) ve
3/Cardenolides
(-) ve
(-) ve
(+) ve
(+) ve
(+) ve
(+) ve
4/Tannin
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
5/Saponins
(+) ve
(+) ve
(-) ve
(-) ve
(-) ve
(-) ve
6/Coumarin
(-) ve
(-) ve
(-) ve
(-) ve
(-) ve
(-) ve
7/Flavonoids
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
8/Steroid
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
9/Carbohydrates
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
(+) ve
Table (2): Alkaloid and tannin content in extracted – tea solvent (water, ethanol and petroleum ether of green and black type.
Type of solvent
Water extraction
Ethanol extraction
Petroleum ether extraction
Type of tea
Green tea
Black tea
Green tea
Black tea
Green tea
Black tea
Alkaloid Mg/g
5.4a±1
6.4 a ±0.5
4.3 a ±0.3
4.5 a ±1.2
4.6 a ±1
5.2 a ±1.4
Tannin %
1.2 a ±0.5
1.2 a ±1
1.8 a ±2
0.5 b ±0.01
1.5 a ±1
1.0 a ±0.8
* Each value is average of three replicates expressed on dry weight basis.
Murwan K. Sabah ELKheir 1
1. Department of Biochemistry, School of Biotechnology, Faculty of Science and Technology, El Neelain University, P.O.Box 12702, Khartoum, Sudan
Email address; murwansabahelkhier@yahoo.com
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